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hbx gene hbx plasmid  (Addgene inc)


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    Addgene inc hbx gene hbx plasmid
    Hbx Gene Hbx Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hbx+gene/ppr0648295-40-2-6?v=Addgene+inc
    Average 92 stars, based on 6 article reviews
    hbx gene hbx plasmid - by Bioz Stars, 2026-07
    92/100 stars

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    MnSOD contributes to decreased mitochondrial superoxide and apoptotic cells in HepG2.215 cells. A: After 6 d, the interference effect of MnSOD siRNA (siMnSOD) was analyzed by Western blot analysis. MnSOD siRNA (siMnSOD) or non-specific siRNA (siCTRL) was transfected into HepG2.215 cells for 12 h before serum depletion; B: After 6 d, cells were harvested for quantification of mitochondrial superoxide anion formation by flow cytometry. In parallel, (C) cell viability and (D) apoptotic cells were separately determined by MTT assay and flow cytometry. aP < 0.05 and bP < 0.01 vs siCTRL group.

    Journal: World Journal of Gastroenterology

    Article Title: Activation of AMPK/MnSOD signaling mediates anti-apoptotic effect of hepatitis B virus in hepatoma cells

    doi: 10.3748/wjg.v22.i17.4345

    Figure Lengend Snippet: MnSOD contributes to decreased mitochondrial superoxide and apoptotic cells in HepG2.215 cells. A: After 6 d, the interference effect of MnSOD siRNA (siMnSOD) was analyzed by Western blot analysis. MnSOD siRNA (siMnSOD) or non-specific siRNA (siCTRL) was transfected into HepG2.215 cells for 12 h before serum depletion; B: After 6 d, cells were harvested for quantification of mitochondrial superoxide anion formation by flow cytometry. In parallel, (C) cell viability and (D) apoptotic cells were separately determined by MTT assay and flow cytometry. aP < 0.05 and bP < 0.01 vs siCTRL group.

    Article Snippet: RNA interference The siRNAs for silencing AMPK, MnSOD and HBx genes as well as scrambled siRNA were purchased from Ribobio (Guangzhou, China).

    Techniques: Western Blot, Transfection, Serum Depletion, Flow Cytometry, MTT Assay

    AMPK activation is required for MnSOD expression in HepG2.215 cells. A: Cell lysates were subjected to Western blot analysis. The protein levels of AMPK and p-AMPK were detected; B: Cells were transfected with AMPK siRNAs (siAMPK) or a nonspecific control siRNA (siCTRL) for 6 d. The protein levels of AMPK and MnSOD were detected by Western blot analysis; C: Cells were subjected to 1 mM AMPK activator AICAR or inhibitor compound C for 6 d. The protein levels of AMPK, p-AMPK and MnSOD were detected by Western blot analysis; D: Cells were transfected with HBx siRNAs (siHBx) or a nonspecific control siRNA (siCTRL) for 6 d. The protein levels of AMPK and MnSOD were detected by Western blot analysis.

    Journal: World Journal of Gastroenterology

    Article Title: Activation of AMPK/MnSOD signaling mediates anti-apoptotic effect of hepatitis B virus in hepatoma cells

    doi: 10.3748/wjg.v22.i17.4345

    Figure Lengend Snippet: AMPK activation is required for MnSOD expression in HepG2.215 cells. A: Cell lysates were subjected to Western blot analysis. The protein levels of AMPK and p-AMPK were detected; B: Cells were transfected with AMPK siRNAs (siAMPK) or a nonspecific control siRNA (siCTRL) for 6 d. The protein levels of AMPK and MnSOD were detected by Western blot analysis; C: Cells were subjected to 1 mM AMPK activator AICAR or inhibitor compound C for 6 d. The protein levels of AMPK, p-AMPK and MnSOD were detected by Western blot analysis; D: Cells were transfected with HBx siRNAs (siHBx) or a nonspecific control siRNA (siCTRL) for 6 d. The protein levels of AMPK and MnSOD were detected by Western blot analysis.

    Article Snippet: RNA interference The siRNAs for silencing AMPK, MnSOD and HBx genes as well as scrambled siRNA were purchased from Ribobio (Guangzhou, China).

    Techniques: Activation Assay, Expressing, Western Blot, Transfection, Control